Assessing Thermal Comfort Perception in the Context of Social Housing. Case Study in Northern Spain

The influence of people on building performance in terms of energy efficiency and environmental impact is becoming increasingly significant. It is essential to include users’ perspective, their comfort and satisfaction in decision making to ensure not only their well-being, but also the feasibility of interventions and the adequate performance of the building stock. Furthermore, understanding residents’ level of thermal satisfaction can enable more appropriate measures to improve the energy efficiency of buildings. Although there are several methods for studying thermal comfort, such as qualitative analyses based on surveys or perceived comfort indices such as PPD and PMV, thermal satisfaction is susceptible to the subjectivity of the responses. It may be necessary to contrast different indices or methods. This study aims to define an indicator that measures the level of thermal satisfaction of social housing occupants so that it can be contrasted with other methods of analysis of perceived comfort and can be replicated in different building contexts. A way to analyse users’ thermal satisfaction is proposed in a quantitative way, measured as the difference of the desired temperature and the perceived indoor temperature. The index is applied to a sample of 283 social housing dwellings in the Basque Country, Spain, with the data obtained via surveys that include questions on thermal comfort in winter and households’ characteristics. The thermal satisfaction has been analysed and the results have been contrasted with the perceived thermal comfort in winter and the household’s capacity to maintain the dwelling at the desired temperature. Moreover, it has been observed whether there may be energy vulnerabilities by contrasting the satisfaction result with the income and expenditure per person in the household. The obtained variable provides occupants’ opinion and perception to ensure the suitability of the solutions for improving the energy efficiency of the building and the thermal comfort. It is also possible to apply it to different building typologies and compare the results with other models of perceived thermal comfort

Occupants’ behavioural diversity regarding the indoor environment in social housing. Case study in Northern Spain

Previous research has shown that differences in preferences, habits, and uses can exist in buildings with similar characteristics, which can influence building performance, energy efficiency, and the well-being of occupants. Among this diversity, those residing in social housing have specific socio-economic and cultural characteristics. This study aims to provide evidence of the diversity of thermal preferences and heating-related behaviours in public social rental housing. It also seeks to develop a methodology for identifying behavioural and occupancy patterns that can be applied in building simulation programs and building stock management. To identify occupancy and heating patterns, quantitative and qualitative data analysis methods were applied. The data was collected from a variety of sources, including sensors and surveys. Advanced statistical methods were used to analyse the data and identify patterns and trends. The study was conducted in 58 dwellings of a public social rental housing building in northern Spain. The results showed a lack of association between perceived and monitored thermal comfort. Additionally, variability in the use of the dwelling has been found among similar socioeconomic profiles. The analysis of behavioural diversity revealed six clusters based on energy consumption behaviour, including occupancy patterns and heating usage. The patterns obtained can be integrated into building performance simulation programs, resulting in a more nuanced and accurate representation of energy consumption patterns. Moreover, these patterns can provide valuable insight into the diversity of energy consumption behaviours. This can be leveraged to unlock new opportunities for energy savings, efficiency gains, and enhancing the well-being of occupants across a variety of use cases.The authors would like to acknowledge the Public Society Alokabide and the company STECHome for their support and contribution, as well as for the information, access and monitoring data provided. This research work will form part of a doctoral theses carried out by S. Perez-Bezos, funded by the Call for tender for a researcher training at the University of the Basque Country UPV/EHU 2019 (PIF19/139). Open Access funding provided by University of the Basque Country

Rare gas systematics on Lucky Strike basalts (37°N, North Atlantic): Evidence for efficient homogenization in a long-lived magma chamber system?

International audienceWe present rare gas data in fresh glasses from the Lucky Strike segment located on the Mid Atlantic Ridge (∼37.3°N), close to the Azores plateau. We analyzed the helium and neon isotopes in 28 samples by melting as well as He‐Ne‐Ar‐Kr‐Xe isotopes in 9 samples by crushing. Samples were collected during the Graviluck06, MOMAR08, and Bathyluck09 cruises over a ridge length of ∼13 km (mean sample spacing of ∼500 m), and at depths ranging from 1550 m to 2174 m. The helium isotopic ratio varies between 84,410 and 88,235 (R/Ra between 8.19 and 8.56). The samples having the “most” primitive helium isotopic ratio are the enriched samples (e.g. high K2O/TiO2) although the difference to the depleted samples is small. It appears that all of our samples derive from the same and well‐homogenized magma chamber. Neon isotopes clearly show the influence of the Azores hotspot, which is not seen with helium because of lower 3He/22Ne in the plume source compared to the MORB source

Assessment of Social Housing Energy and Thermal Performance in Relation to Occupants & Behaviour and COVID-19 Influence: A Case Study in the Basque Country, Spain

Evidence shows that people have a major impact on building performance. Occupants’ impact is especially important in social housing, where their occupants may present greater vulnerabilities, and their needs are not always considered. This study aims to analyse the socio-demographic influence in social rental housing concerning hygrothermal comfort and energy consumption in a case study located in Vitoria, Spain during the first 4-month period of 2020 and 2021 (during and after COVID-19 lockdown). An innovative data management system is included, where the users and administration can see in real-time the temperature and consumption in the dwellings. A 2-phase method has been applied; phase 1 is associated with outdoor climate conditions, building properties and social profile. Phase 2 determined the results in energy consumption, indoor hygrothermal comfort and occupant energy-use pattern. The results show that the comfort levels and energy consumption vary according to the analysed social profiles, as well as the heating activation periods and domestic hot water system usage. In conclusion, socio-demographic characteristics of social housing households influence the hygrothermal comfort of their dwellings, occupants’ behaviour and heating and domestic hot water energy consumption.This research was funded by the research project “Development of a methodology with a life cycle perspective to evaluate the energy rehabilitation actions of the existing building stock in accordance with the requirements of the new technical code” (PUE_2020_1_0013), supported by the Department of Education of the Basque Government

Validation of a Real-Time PCR for the Detection of Mycobacterium tuberculosis Complex Members in Bovine Tissue Samples

Although the post-mortem diagnosis of bovine tuberculosis is mainly achieved through microbiological culture, the development of other techniques to detect Mycobacterium tuberculosis complex (MTBC) members directly from tissue samples has been pursued. The present study describes the development, optimization and validation of a Real-Time PCR based on the mpb70 gene to detect MTBC members in clinical tissue samples from cattle. Specific primers and a hybridization probe were used to amplify MTBC-specific sequences in order to avoid cross-reaction with non-MTBC species. An Internal Amplification Control (IAC) was included in order to assess the presence of PCR inhibitors in the samples. The PCR was optimized to achieve maximum efficiency, and the limit of detection, limit of quantification and dynamic range of the reaction were determined. The specificity of the reaction was tested against 34 mycobacterial and non-mycobacterial species. The diagnostic sensitivity, specificity and positive and negative predictive values (PPV and NPV) of the method were assessed on 200 bovine tissue samples in relation to bacteriological culture. The dynamic range of the reaction spanned from 5 ng/reaction (106 genome equivalents) to 50 fg/reaction (10 genome equivalents). The efficiency of the reaction was 102.6% and the achieved R2 was 0.999. The limit of detection with 95% confidence was 10 genome equivalents/reaction. No cross-reactions with non-MTBC species were observed. The diagnostic sensitivity and specificity values of the mpb70 specific Real-Time PCR respect to culture were 94.59% (95% CI: 86.73–98.51%) and 96.03% (95% CI: 90.98–98.70%), respectively, with a PPV of 93.33% (95% CI: 85.55–97.07%) and a NPV of 96.80% (95% CI: 92.10–98.74%). The concordance of the Real-Time PCR based on mpb70 is comparable to that of culture (K = 0.904) showing a great potential for the detection of members of the MTBC in animal tissues

Effect of the Inoculation Site of Bovine and Avian Purified Protein Derivatives (PPDs) on the Performance of the Intradermal Tuberculin Test in Goats From Tuberculosis-Free and Infected Herds

The single and comparative intradermal tuberculin (SIT and CIT) tests are used for the ante-mortem diagnosis of caprine tuberculosis (TB). The tuberculin injection site has been associated with a different performance of the test in cattle. In contrast to that required in cattle in Europe (cervical injection), it can be carried out in the scapular region in goats. Nevertheless, there are no previous data concerning the effect of the injection site on the performance of the test in goats. The aim of the present study was to evaluate the effect of two different inoculation sites (cervical and scapular) on the performance of the SIT/CIT tests. This was done by intradermally inoculating 309 goats from two infected herds and one TB-free herd with both avian and bovine PPDs in the mid-cervical and scapular regions. None of the animals from the TB-free herd had positive reactions, and the number of reactors was not significantly higher, regardless of the inoculation site, in the high and low prevalence herds. However, significantly higher increases in skin fold thickness were observed on the cervical site when compared to the scapular site after the avian and bovine PPD inoculations in the TB-free herd (p < 0.001) and after the bovine PPD injection in the high prevalence herd (p = 0.003). The presence of clinical signs was also more evident on the cervical site when using avian and bovine PPDs in the high prevalence herd (p < 0.01). In contrast, increases in higher skin fold thickness were observed on the scapular site when compared to the cervical site after the bovine and avian PPD inoculations were employed in the low prevalence herd (p < 0.01). These results suggest that the cervical injection of PPDs may improve the sensitivity of the intradermal tuberculin test in high TB prevalence caprine herds, mainly owing to the increased presence of local clinical signs and a better performance of the CIT test. Moreover, specificity was not affected when using standard interpretations, although further analyses in a great number of herds are required in order to confirm these findings.This study was funded by the Herramientas para alcanzar la erradicación de la tuberculosis caprina (GoaTBfree) project (PID2019-105155RB-C31) and the Spanish Government's Ministerio de Agricultura, Pesca y Alimentación. JO was supported by an FPU (Formación de Profesorado Universitario) contract-fellowship provided by the Spanish Ministerio de Ciencia, Innovación y Universidades (FPU18/05197).S

Evaluation of P22 ELISA for the Detection of Mycobacterium bovis-Specific Antibody in the Oral Fluid of Goats

The ante-mortem diagnosis of tuberculosis (TB) in ruminants is based mainly on the intradermal tuberculin test and the IFN-γ assay. Antibody (Ab)-based tests have emerged as potential tools for the detection of TB infected animals using serum, plasma, or even milk samples. Oral fluids have also been evaluated as alternative samples with which to detect specific Abs against Mycobacterium bovis in pigs or wild boars, but not in ruminants. The objective of this study was, therefore, to evaluate the performance of an in house-ELISA for TB diagnosis (P22 ELISA) in goats as an experimental model for the diagnosis of TB using oral fluid samples. Oral fluid samples from 64 goats from a TB-infected herd (n = 197) and all the animals from a TB-free herd (n = 113) were analyzed using the P22 ELISA. The estimated sensitivity (Se) and specificity (Sp) were 34.4% (95% CI: 22.4-45.6) and 100% (95% CI: 97.4-100), respectively. The optimal cut-off point was set at 100% according to the ROC analysis. Those animals with a higher level of Abs in their oral fluid attained a higher lesion score (p = 0.018). In fact, when taking into account only the setting of the animals with severe lesions (n = 16), the ELISA showed a Se of 75% (95% CI: 53.7-96.2). Results of the present study suggest that the P22 ELISA is highly specific but has a limited value detecting infected animals in oral fluid samples. Nevertheless, its performance is significantly higher in the presence of severe lesions.This study was funded by the Herramientas para alcanzar la erradicación de la tuberculosis caprina (GoaTBfree) project (PID2019-105155RB-C31) and the Spanish Government's Ministerio de Agricultura, Pesca y Alimentación. JO was supported by an FPU (Formación de Profesorado Universitario) contract-fellowship provided by the Spanish Ministerio de Ciencia, Innovación y Universidades (FPU18/05197).S

Wild boar tuberculosis in Iberian Atlantic Spain: a different picture from Mediterranean habitats

BACKGROUND Infections with Mycobacterium bovis and closely related members of the Mycobacterium tuberculosis complex (MTC) are shared between livestock, wildlife and sporadically human beings. Wildlife reservoirs exist worldwide and can interfere with bovine tuberculosis (TB) eradication efforts. The Eurasian wild boar (Sus scrofa) is a MTC maintenance host in Mediterranean Iberia (Spain and Portugal). However, few systematic studies in wild boar have been carried out in Atlantic regions. We describe the prevalence, distribution, pathology and epidemiology of MTC and other mycobacteria from wild boar in Atlantic Spain. A total of 2,067 wild boar were sampled between 2008 and 2012. RESULTS The results provide insight into the current status of wild boar as MTC and Mycobacterium avium complex (MAC) hosts in temperate regions of continental Europe. The main findings were a low TB prevalence (2.6%), a low proportion of MTC infected wild boar displaying generalized TB lesions (16.7%), and a higher proportion of MAC infections (4.5%). Molecular typing revealed epidemiological links between wild boar and domestic - cattle, sheep and goat - and other wildlife - Eurasian badger (Meles meles) and red fox (Vulpes vulpes) - hosts. CONCLUSIONS This study shows that the likelihood of MTC excretion by wild boar in Atlantic habitats is much lower than in Mediterranean areas. However, wild boar provide a good indicator of MTC circulation and, given the current re-emergence of animal TB, similar large-scale surveys would be advisable in other Atlantic regions of continental Europe